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The condensers of various upright and inverted microscopes. The positions of irises and other components vary depending on the manufacturer and model. A. Field diaphragm. The DM2500 field diaphragm is on the other side of the stand. The Nikon Ti field diaphragm is near the top of the transmitted light pillar, labelled F B. Condenser focus C. Centring screws. The Leica DM2500 and DMI6000 have to be centred using hexagonal screwdrivers or Allen keys D. Iris aperture. The iris aperture on the DMI6000 is adjusted using the AP buttons on the left of the stand |
Alignment Procedure
Focus on the specimen using a low power lens like a 10x air. Fully close the field diaphragm and adjust the condenser focus so the image of the iris is in sharp focus. Centre the image of the iris using the two centring screws. Open the field diaphragm until it is just outside the field of view. Remove an eyepiece and look into the empty opening. Adjust the iris aperture until it is about 70% open and then replace the eyepiece. The microscope is now adjusted for Köhler illumination.
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