...
- For more advanced image acquisition configurations with multiple positions set up your channels as described in SIMPLE CONFOCAL IMAGE ACQUISITION. Then open the Multi-Area Time-Lapse controller, which can be found under Device -> Multiarea Time-Lapse.
- An XY-stage calibration warning will open. Press OK to calibrate the stage, which is important to be able to register points for multipoint acquisitions.
- If you would like to use the Z-Drift Controller (ZDC) to keep the focus stable throughout the experiment follow the instruction in the Z-DRIFT CORRECTION (ZDC) section. Be aware that you will need to restart the software to set this up.
- In Multiarea Time-Lapse Controller:
- 1st button from left: Add current position (to save a position, then move on to next -> gives you list of data points in Registered Point List)
- 2nd button from left: Mosaic Outline (to select separate points and then fills in gaps between them to scan an area)
- 3rd button from left: Define Matrix (specify central point and define area around it)