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1. For more advanced image acquisition configurations with multiple positions set up your channels as described in SIMPLE CONFOCAL IMAGE ACQUISITION. Then open the Multi-Area Time-Lapse controller, which can be found under Device -> Multiarea Time-Lapse.
   
   
2. An XY-stage calibration warning will open. Press OK to calibrate the stage, which is important to be able to register points for multipoint acquisitions.
   
   
3. If you would like to use the Z-Drift Controller (ZDC) to keep the focus stable throughout the experiment follow the instruction in the Z-DRIFT CORRECTION (ZDC) section. Be aware that you will need to restart the software to set this up.
   
   
4. In Multiarea Time-Lapse Controller:
   1. 1^st button from left: Add current position (to save a position, then move on to next -> gives you list of data points in Registered Point List)
   2. 2^nd button from left: Mosaic Outline (to select separate points and then fills in gaps between them to scan an area)
   3. 3^rd button from left: Define Matrix (specify central point and define area around it)
      
      
5. Go to View -> Stage controller to control the stage using the software or use the manual XY-controller to navigate to different positions in your sample and create a point map using the Add Current Position, Mosaic Outline or Define Matrix method. 
   
   
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