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In the ND Acquisition window tick the Save to File box and define a filename and path for the timelapse (see image below).
Setting up fluorescence and transmitted light channels
Click the lambda tab and select the channels you want to image by ticking the box at the start of each row and selecting from the drop-down list of optical configurations (red arrow below). Tick the box at the bottom of the window that says ‘Close active Shutter during Filter Change’ if you want to reduce photobleaching.
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