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Setting up the length of the timelapse and frequency of acquisition
Click the Timing… button to check how long a single loop will take; this will determine the minimum Interval between time points. Click the Time tab and select at least one tick box at the start of a row. Specify the desired Interval and a Duration or number of Loops. Ticking a box in the row below will create another time Phase, so for instance you can have a fast acquisition for a short period followed by a slow acquisition over a longer period. Click the ‘Close Active Shutter when Idle’ box to reduce photobleaching.
Click ‘1 1 time loop’ to loop to test the settings or ‘Run Now’ Run Now to begin the timelapse.