...
Deep Thought is Windows 7 64-bit image analysis PC with dual Intel XEON CPU E5-2620 2 GHz processors (6 cores 12 logical partitions), 160 GB RAM and an AMD Radeon HD 7800 2 GB VRAM graphics card. This computer has almost the maximum amount of RAM possible in Windows 7, which makes it very suitable for memory intensive tasks like image deconvolution. The deconvolution software package Huygens Essential is installed on this machine to make best use of the RAM. There is also a full copy of Carl Zeiss's Zen software installed (both Black and Blue editions) with a module for processing Airyscan data offline and a copy of Nikon NIS-Elements with an offline package for analysing NSTORM data.
...
Software on Deep Thought
Huygens Essential
Compatible with Windows Remote Desktop (RDP)
...
I find that the Restoration module is not great at deconvolving data with lower signal to noise ratios, so I tend to use Huygens and AutoQuant for deconvolution. It is difficult to set up an analysis pipeline using the Quantitation but all such tools are difficult and require a lot of trial and error, so this isn't unusual. The analysis and charts tools in the Quantitation module are not intuitive at all so most of the time I export data as a spreadsheet for analysis in other packages (e.g. Excel). The Tracking tools for measuring objects moving over time seem to be a lot more flexible and user friendly in Imaris.
...
Free image analysis software and browsers
...
Fiji (ImageJ)
The Fiji installed on this computer currently has a number of locally installed modules in addition to the standard Fiji plugins that are automatically updated. Here are a few of the plugins available.
...