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In most cases you only have permission to book those machines on which you have been trained. Click the Permissions tab on the right to see the microscopes and computers you can use. Click a resource to open its calendar.
Each resource has its own calendar (e.g. in this case, GFP3). The Fields tab varies between resources but can allow you to do such things as specify the required temperature for an environmental chamber. In the Options tab you can report incidents (e.g. if the software or hardware was not working properly during your booking).
To book a slot, just drag the mouse downwards to adjust the the length of the time slot. You can drag overnight to the following morning but not from Sunday to Monday. Right-clicking on your booked slot opens a list (indicated by a red arrow in the image), which allows you to modify your booking. For example, you can delete your booking or request assistance from a manager if you need help setting up the microscope for your experiment. You can also right-click on someone else's slot and select Waiting List. If the slot becomes available you will receive an automatic notification.