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To acquire images, select Acquire and the Acquisition tab.
- You can save the correct objective into your image metadata by selecting the magnification you are using (under Magnification). As the turret is manually operated the objectives are changed by hand. There is also a 1.5x magnification changer that can be put into the light path using the wheel on the right of the microscope stand and changing the "Magnification Changer" drop down selection. Make sure to let the software knows whether the 1.0x or 1.5x lens is in place, otherwise your image scaling will be wrong.
- To see a live view image, click 'Live'; to take an image, click 'Capture Image'.
- Select colour or black and white mode by using the selectors. Note : We advise using black and white mode for all fluoresecently stained specimens, and merging them later in post processing.
Taking colour images
- Select Color Mode in the software.
- Set binning to 1x1 for both Image and Live Formats.
- Set the exposure to 1 ms.
- Insert both the N4 and N16 neutral density (ND) filters into the light path and turn the lamp voltage up to 11 or 12. By turning up the lamp and inserting the ND filters the light from the lamp should be the right colour and brightness for your eyes and the camera.
- Focus on your specimen and redirect the light path to the camera port.
- Click Live and adjust the camera exposure to avoid saturation.
- For brightfield images, set the white balance using the "draw rectangle" tool and selecting 'white balance' when the options appear.
- Set the exposure time in the imaging tab. Leave the Gain at 1.
- To save, go to 'Open projects', right click on your file or folder, and save. LAS X will save whole folders of images into one single lif. If you want individual tifs, use the right click > export option.
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