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Wide-field Fluorescence Filters
Filter These filter and dichroic mirror configurations are available when the light path is oriented towards the fluorescence filter wheel and Andor Zyla camera on the right-hand port.
Sedat quadQuad-band dichroic and single-band emission filters for slower switching. Lower speed but reduced filters for fast switching to the Zyla Camera. High speed but risk of cross-talk between channels.
Filter ID | Excitation Wavelength | Dichroic Mirror | Emission Filter (in |
|---|
quad) | Example Fluorophores |
|---|---|
| DAPI | 385 nm |
| 86012v2 Dapi/FITC/TxRed/Cy5 | ~ 455nm - 485nm | DAPI, Hoechst |
| FITC | 470 nm |
| 86012v2 Dapi/FITC/TxRed/Cy5 | ~ 510nm - 545nm | GFP, FITC |
| TxRed | 550 nm |
| 86012v2 Dapi/FITC/TxRed/Cy5 | ~ 590nm - 630nm | RFP, mCherry |
| Cy5 |
| 635 nm |
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| 86012v2 Dapi/FITC/TxRed/Cy5 | ~ 670nm - 740nm | Cy5 |
Sedat quad-band dichroic and single-band emission filters for slower switching. Lower speed but reduced cross-talk between channels.
Filter ID | Excitation Wavelength | Dichroic Mirror | Emission Filter (in |
|---|
wheel) | Example Fluorophores |
|---|---|
| DAPI | 385 nm |
| 89000 Sedat Quad | 435 - 485 | DAPI, Hoechst |
| GFP | 470 nm |
| 89000 Sedat Quad | 500 - 550 | GFP, FITC |
| RFP | 550 nm |
| 89000 Sedat Quad | 590 - 650 | RFP, mCherry |
| Cy5 |
| 660 nm | 89000 Sedat Quad | 662 - 737 | Cy5 | |
| CFP | 470 nm | 89002 CFP/YFP | 460 - 500 | CFP |
| eYFP | 500 nm | 89002 CFP/YFP | 520 - 550 | YFP |
iSIM Filters
The iSIM has the following quad-band emission filter in its scan head:
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