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The iSIM has the following quad-band dichroic and emission filter in its scan head:. The emission filter is Chroma part number 88100bs from Pinkel set 88000v2.
| Laser | Dichroic Mirror (reflection bands) | Emission Filter | Example Fluorophores |
|---|---|---|---|
| 405 | 400 - 410 | 430 - 485 | DAPI, Hoechst |
| 488 | 483 - 493 | 520 - 550 | GFP, FITC |
| 561 | 556 - 566 | 590 - 630 | RFP, mCherry |
| 640 | 635 - 645 | 680 - 740 | Cy5 |