...
The iSIM has the following quad-band dichroic and emission filter in its scan head. The emission filter is Chroma part number 88100bs from Pinkel set 88000v2.
Laser | Dichroic Mirror (reflection bands) | Emission Filter | Example Fluorophores |
---|---|---|---|
405 | 400 - 410 | 430 - 485 | DAPI, Hoechst |
488 | 483 - 493 | 520 - 550 | GFP, FITC |
561 | 556 - 566 | 590 - 630 | RFP, mCherry |
640 | 635 - 645 | 680 - 740 | Cy5 |
Camera Specifications
- Zyla 4.2 Plus specifications (Andor website)
- Hamamatsu Orca Flash4.0 v3 specifications (Hamamamatsu website)