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- STED is only possible using the specially optically corrected 100x HC PL APO CS2 oil lens, so you must first rotate this into position in the nose piece by selecting it in the drop-down menu in the UI.
- After the 100x lens is in position you can click the Activate STED button in the top right of the left control screen. The Activate STED button will be greyed out until the 100x lens is in position.
- At this stage you may see a warning prompting you to carry out a STED beam alignment procedure. This is an automatic process carried out in the Configuration tab that you should carry out You should do this even if you are not prompted to do so because in my experience the lasers can become badly misaligned over time and the beam alignment procedure has fixed that.
- Go to Configuration and click the STED button.
- In the STED configuration window, click Align Beams.