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Switch on the PC Microscope, Scanner Power and Laser Power buttons and turn the Laser Emission key from Off to On. Wait for the computer to boot up, login to the account and then turn on the depletion lasers. These are on the power supply to the left of the microscope, labelled 775-nm, 660-nm and 592-nm. The switches should always be left in the on position, but the keys will need to be turned one quarter-turn clockwise to the Laser On position.
Software start-up
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