If necessary, boot up the laptop computer and login to the ONI account.
Double-click the NimOS icon on the desktop to launch the acquisition and analysis software.
Click the Acquire button in the top left and then click Connect to Microscope.
NimOS will open the dialogue box below asking you to check the stage before it connects to the device to prevent damage.
Push the interlock slider to the in position and lift the lid of the chamber to make sure the area around the stage is clear and that all samples have been removed. There should be two stacks of small disc magnets, one stack of three on either side of the stage. They are there to secure the specimen.
Leave the lid open and click Confirm and Connect. There will be noise as the laser engine starts up and messages will appear in NimOS as the computer connects to the different components.
You will see that the stage moves to very high position as part of its start-up. This is why it needs to be clear prior to initialisation.
In the Temperature Control area on the left, set the Target Temperature to 31ºC or 37ºC and press the Enable Control button (the button says Disable Control once pressed). The Nanoimager operates at a temperature around 6 degrees warmer than ambient and the temperature control must be set slightly above that to maintain stability. This is something to take into consideration when planning applications where temperature is important.
Once the temperature has stabilised the control can be disabled.
Note
ONI say that it will take the microscope 60 to 90 minutes for the microscope to reach 37°C and stabilise.