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  1. The best way start acquiring data is to use multidimensional acquisition. Click the Multiple Acquisition Setup button under the focus camera view on the left.
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  2. In the Acquisition tab, click Single, set imaging mode to Normal, specify adjust the laser power and make sure the button above the percentage slider is clicked (text is white, exposure/frequency colour is bright). Set the exposure and number of images.
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  3. Ignore the Priority and AutoFocus and Z Reference tabs. They are not important for conventional 2D dSTORM at a single stage position.
  4. In Saving, specify the Dataset Tag, which will be the folder where the files are stored, and the Acquisition Tag, which will be the filename. Note that the date will be automatically included in the filename.

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