...
- The best way start acquiring data is to use multidimensional acquisition. Click the Multiple Acquisition Setup button under the focus camera view on the left.
- In the Acquisition tab, click Single, set imaging mode to Normal, adjust the laser power and make sure the button above the percentage slider is clicked (text is white, colour is bright). Set the exposure and number of images.
- Ignore the Priority and AutoFocus and Z Reference tabs. They are not important for conventional 2D dSTORM at a single stage position.
- In Saving, specify the Dataset Tag , which will be the folder where the files are stored, and the Acquisition Tag , which will be the filename. Note that the date (see Acquisition Control above for an explanation). If you are logged into your user login all files will be automatically included saved in the filenameyour folder.
- Click the Start button at the bottom to begin the acquisition.
You can get more information about multidimensional acquisition in the Knowledge Base on the ONI website.