Page Comparison

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1. Click View in the Acquisition Control area to get begin a live feed from the acquisition camera.
   
2. There will be two live images, which correspond to two simultaneous colour channels. For dSTORM using a far-red dye you only need Channel 1, so untick Channel 0 in the Image Display Options on the left. For dyes other emitting wavelengths lower than far-red you will need Channel 0.
   
3. The live camera image will only show noise until you illuminate the sample with a laser. Change the percentage power in the Light Control area by either clicking on the spin buttons or typing in a value and pressing the Return key. The laser power in the image below is set to 100% but it is best to use a lower power like 10% to avoid photobleaching while setting up your specimen. Click on the button above the laser (e.g. red 640 button) to switch on the illumination.
   
4. Focus on your specimen. If you are using Z lock you may need to adjust the Z offset as explained in the section on focusing.
5. Turn Once you're in focus turn off the laser when not imaging so you don’t photobleach the specimen.

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In the Acquisition Control area on the right at the bottom of the user interface you can define the exposure and number of images to be captured, as well as the naming scheme for the files.

Image Added

The Dataset tag is the name of the top-level folder that is created to hold all image files captured during that session. A date stamp is automatically added to the folder name so there is no need to include the date in the name. Each individual acquisition during a session is named according to the text entered into the Acquisition tag field. If you created a user login, all the acquired files and folders will be saved in your own folder. the View button begins a live camera preview while the Acquire button will start imaging using the exposure and number of images set within the Acquisition Control area.

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Multiple Acquisition Setup

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