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The settings in the following guide are appropriate for single-channel 2D dSTORM of a sample labelled with a far red dye like Alexa 647. Your sample or procedure may require other settings, such as an additional imaging channel, different laser wavelengths and powers, or 3D acquisition. If you need to use different settings, then you can either look for more information on the wiki or in ONI's knowledgebase or contact the light microscopy email: lmcb-lm-help@ucl.ac.uk.
- Click the Multiple Acquisition Setup button under the focus camera view on the left.
- In the Acquisition tab, click Single, set imaging mode to Normal, adjust the 635 laser power to 100% and make sure the button above the percentage slider is clicked to activate the laser (text is white, colour is bright). Set the exposure and number of images.
- Ignore the Priority and AutoFocus and Z Reference tabs. They are not important for conventional 2D dSTORM.
- In the Saving tab, specify the Dataset Tag and the Acquisition Tag (see Acquisition Control above for an explanation of the tags). If you are logged into your User login all files will be automatically saved in your folder.
- Click the Start button at the bottom to begin the acquisition.
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