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The settings in the following guide are appropriate for single-channel 2D dSTORM of a sample labelled with a far red dye like Alexa 647. Your sample or procedure may require other settings, such as an additional imaging channel, different laser wavelengths and powers, or 3D acquisition. If you need to use different settings, then you can either look for more information on the wiki or in ONI's knowledgebase (login required) or contact the light microscopy email: lmcb-lm-help@ucl.ac.uk.
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