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In the Acquisition Control area on the right at the bottom of the user interface you can define the exposure and number of images to be captured, as well as the naming scheme for the files. The Dataset tag is the name applied to a top-level folder that is created to hold all the image files captured during that session. A date stamp is automatically added to the folder name so there is no need to include the date in the name. Each individual acquisition during a session is named according to the text entered into the Acquisition tag field. If you've logged into your user login, all the acquired files and folders will be saved in your folder. The View button begins a live camera preview, which you can use while focusing or otherwise adjusting your acquisition settings. The A and B buttons allow you to create two sets of acquisition conditions.
To set up an acquisition for single-channel 2D dSTORM of a sample labelled with a far red dye like Alexa 647:
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