- Click on the Analyze tab.
- If you have just acquired a dataset it will already be listed in the Image Files: list.
- To load a new dataset, click Load Data...
By default NimOS will localise molecules in real time during the acquisition, in which case the Result Files: list will have a locb and nimb file in it.
Note If Disable real-time localization was ticked prior to the acquisition then the Result Files list will be empty.
- In the Viewing Options area in the lower right of the user interface you can tick or untick the raw or Localizations columns to display the raw image data and/or localisation image.
- Double-clicking the block of colour in the Colour column opens a dialogue box that will allow you to change the colour and rendering.
- If the Raw column is ticked you can scroll through the Frame index of the acquisition sequence.
- In the Results Filtering area you can choose to apply filters to the localisations. For example, you might want to only look at localisations with a localisation precision of 10 nm or less, or you might want to examine how the localisations change for different ranges of frames using the Frame Index filter.
- On the left of the user interface in the Tools area you can use the Trace tool to see how parameters like photon count change over time or use the Line Histogram to measure the distance between spots.
- Localisations can be exported using the Export Localizations as .csv button. If you've set filters in the Results Filtering area then the filtered localisations will be exported.
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