Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.
  1. Click on the Analyze tab.
  2. If you have just acquired a dataset it will already be listed in the Image Files: list.
  3. To load a new dataset, click Load Data...
  4. By default NimOS will localise molecules in real time during the acquisition, in which case the Result Files: list will have a locb and nimb file in it.

    Note

    If Disable real-time localization was ticked prior to the acquisition then the Result Files list will be empty.

Viewing Options

The information below relates to single-colour dSTORM. Multi-colour data would have more channels, each in a different row below Channel 0.

  1. In the Viewing Options area in the lower right of the user interface you can tick or untick the raw or Localizations columns to display the raw image data and/or localisation image.
  2. Double-clicking the block of colour in the Colour column opens a dialogue box that will allow you to change the colour and rendering.
  3. If the Raw column is ticked you can scroll through the Frame index of the acquisition sequence.

...