- Click on the Analyze tab.
- If you have just acquired a dataset it will already be listed in the Image Files: list.
- To load a new dataset, click Load Data...
By default NimOS will localise molecules in real time during the acquisition, in which case the Result Files: list will have a locb and nimb file in it.
Note If Disable real-time localization was ticked prior to the acquisition then the Result Files list will be empty.
Viewing Options
The information below relates to single-colour dSTORM. Multi-colour data would have more channels, each in a different row below Channel 0.
- In the Viewing Options area in the lower right of the user interface you can tick or untick the raw or Localizations columns to display the raw image data and/or localisation image.
- Double-clicking the block of colour in the Colour column opens a dialogue box that will allow you to change the colour and rendering.
- If the Raw column is ticked you can scroll through the Frame index of the acquisition sequence.
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