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  1. In the Viewing Options area in the lower right of the user interface you can tick or untick the Raw or Localizations columns to display the raw image data and/or localisation image (or both superimposed on one another).
  2. Double-clicking the block of colour in the Colour column opens a dialogue box that will allow you to change the colour and rendering for each channel..
  3. If the Raw column is ticked you can scroll through the Frame index of the acquisition sequence.

Drift Correction

There is a Calculate Drift Correction button amongst the Tools in the bottom left of the UI.

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This works best when calculating drift from dense datasets with large numbers of localisations as the default minimum number of points to cross-correlate is 400000. For sparser datasets ONI have written a Python script that changes the default number to a user-set value.

Results Filtering

  1. In the Results Filtering area you can choose to apply filters to the localisations. For example, you might want to only look at localisations with a localisation precision of 10 nm or less, or you might want to examine how the localisations change for different ranges of frames using the Frame Index filter.
  2. On the left of the user interface in the Tools area you can use the Trace tool to see how parameters like photon count change over time or use the Line Histogram to measure the distance between spots.
  3. Localisations can be exported using the Export Localizations as .csv button. If you've set filters in the Results Filtering area then the filtered localisations will be exported.