Super-Resolution Microscopy
Glox Switching Buffer
The Glox buffer formulation is adapted from the following publication:
To make 2.2 ml final volume, which will fill one well of a 4-well iBidi ibidi µ-Slide.
- 110 µl Solution A
- 880 µl Solution B
- 220 µl Solution C
- 990 µl PBS
Solution A (Enzyme Solution)
- 10 µl catalase
- 20 µl 1M TCEP
- 2 ml glycerol
- 125 µl 1M KCl
- 100 µl 1M Tris-Cl pH 7.5
- 50 mg glucose oxidase
- Top up to 5 ml with distilled water
Dispense into 50 µl aliquots and store frozen at -20°C.
Solution B (Glucose Solution)
- 4 g glucose
- 4 ml glycerol
- 36 ml distilled water
Dispense into 400 µl aliquots and store at -20°C.
Solution C (Reducing Agent Solution)
- 113.6 mg MEA-HCl
- 1 ml distilled water
Use fresh or make 100 µl aliquots for storage at -20°C