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| Standard Objective Lenses | |||||
| Magnification | Type | Immersion | NA | DIC | WD (mm) |
|---|---|---|---|---|---|
| 10x | Plan Fluor | Air | 0.3 | - | 16 |
| 20x | Plan Fluor | MImm | 0.75 | M | 0.33 - 0.35 |
| 40x | Plan Fluor | Oil | 1.3 | H | 0.2 |
| 60x | Plan Apo | Oil | 1.4 | H | 0.21 |
| Water Dipping Lenses | |||||
| Magnification | Type | Immersion | NA | DIC | WD (mm) |
| 20x | Fluor | Water | 0.5 | M | 2 |
| 40x | Fluor | Water | 0.8 | M | 2 |
| 60x | Fluor | Water | 1.0 | H | 2 |
The letters in column 5 indicate the correct prism for use with the lens | |||||
Lasers
| Argon laser |
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| Line | Fluorophores | Notes |
|---|---|---|
| 458 | CFP | This is not a very strong line |
| 476 | - | Could be used as an alternative for green dyes |
| 488 | EGFP, FITC, Alexa 488 | |
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| 514 | YFP and related proteins |
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| Green HeNe | ||
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| Line | Fluorophores | Notes |
|---|---|---|
| 543 | TRITC, Alexa 532, Alexa 546, Alexa 555, Cy3 | |
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| Can also be used for 'redder' dyes like Texas Red, Alexa 568, Alexa 594, mCherry but with lower excitation efficiency | ||
| Red Diode | ||
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| 637 | Cy5, Alexa 633, Alexa 647, silicon rhodamine dyes, DRAQ5 | |
Note that there is no 405 nm laser on this machine so you cannot image Hoechst, DAPI or anything else in the blue region of the visible spectrum
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