...
| Standard Objective Lenses | |||||
| Magnification | Type | Immersion | NA | DIC | WD (mm) |
|---|---|---|---|---|---|
| 10x | Plan Fluor | Air | 0.3 | - | 16 |
| 20x | Plan Fluor | MImm | 0.75 | M | 0.33 - 0.35 |
| 40x | Plan Fluor | Oil | 1.3 | H | 0.2 |
| 60x | Plan Apo | Oil | 1.4 | H | 0.21 |
| Water Dipping Lenses | |||||
| Magnification | Type | Immersion | NA | DIC | WD (mm) |
| 20x | Fluor | Water | 0.5 | M | 2 |
| 40x | Fluor | Water | 0.8 | M | 2 |
| 60x | Fluor | Water | 1.0 | H | 2 |
The letters in column 5 indicate the correct prism for use with the lens | |||||
Lasers
| LineLine | Laser | Fluorophores | Notes |
|---|---|---|---|
| 458 | Argon | CFP | This is not a very strong line |
| 476 | Argon | - | Could be used as an alternative for green dyes |
| 488 | Argon | EGFP, FITC, Alexa 488 | |
| 514 | Argon | YFP and related proteins | Green HeNe |
| 543 | |||
| Line | Fluorophores | Notes | |
| 543 | GreenHeNe | TRITC, Alexa 532, Alexa 546, Alexa 555, Cy3 | Can also be used for 'redder' dyes like Texas Red, Alexa 568, Alexa 594, mCherry but with lower excitation efficiency |
| 637 | Red Diode | ||
| 637 | Cy5, Alexa 633, Alexa 647, silicon rhodamine dyes, DRAQ5 |
...