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Standard Objective Lenses | |||||
Magnification | Type | Immersion | NA | DIC | WD (mm) |
---|---|---|---|---|---|
10x | Plan Fluor | Air | 0.3 | - | 16 |
20x | Plan Fluor | MImm | 0.75 | M | 0.33 - 0.35 |
40x | Plan Fluor | Oil | 1.3 | H | 0.2 |
60x | Plan Apo | Oil | 1.4 | H | 0.21 |
Water Dipping Lenses | |||||
Magnification | Type | Immersion | NA | DIC | WD (mm) |
20x | Fluor | Water | 0.5 | M | 2 |
40x | Fluor | Water | 0.8 | M | 2 |
60x | Fluor | Water | 1.0 | H | 2 |
The letters in column 5 indicate the correct prism for use with the lens |
Lasers
Line | Laser | Typical Fluorophores | Notes |
---|---|---|---|
458 | Argon | CFP | This is not a very strong powerful laser line |
476 | Argon | - | Could be used as an alternative for green dyes when trying to separate dyes with similar spectra |
488 | Argon | EGFP, FITC, Alexa 488 | |
514 | Argon | YFP and related proteins | Could also be used for Citrine, Venus, etc |
543 | GreenHeNe | TRITC, Alexa 532, Alexa 546, Alexa 555, Cy3 | Can also be used for 'redder' dyes like Texas Red, Alexa 568, Alexa 594, mCherry but with lower excitation efficiency |
637 | Red Diode | Cy5, Alexa 633, Alexa 647, silicon rhodamine Si Rhodamine dyes, DRAQ5 |
Note that there is no 405 nm laser on this machine so you cannot image Hoechst, DAPI or anything else in the blue region of the visible spectrum
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