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Note that at 1x binning the camera is in colour mode and the red, green and blue colour balancing sliders are active. In 2x binning the camera is greyscale.
Colour Balancing
Colour images (e.g. of histologically stained specimens) must be white balanced so the halogen lamp does not impart a yellow or blue tint to the image. The procedure for white balancing a bright field image is as follows:
- Focus on your specimen and ensure that the illumination is correctly aligned
- Insert the two neutral density (ND) filters into the light path by pressing in the two front-most buttons on the right-hand side of the stand
- Turn the lamp brightness up to full (12 volts) and direct the light to the camera
- Adjust the exposure of the image so that there is no saturation
- Find an area that should be white and draw a marquee in that area
- Right-click on the image and select Auto White Balance
For fluorescent images the white balance must be reset. Right-click the image and select Reset White Balance. Both of these white balancing tools can also be found in the Image menu.