...
Most
...
microscopes
...
are
...
widefield
...
microscopes.
...
In
...
widefield
...
microscopy
...
the
...
entire
...
field
...
is
...
simultaneously
...
illuminated
...
throughout
...
its
...
full
...
width
...
and
...
depth
...
with
...
the
...
...
...
...
of
...
the
...
objective
...
lens
...
determining
...
how
...
much
...
of
...
the
...
specimen
...
appears
...
in
...
focus.
...
Imaging
...
and
...
processing
...
of
...
single
...
planes
...
can
...
be
...
quick
...
and
...
easy
...
because
...
an
...
entire
...
field
...
can
...
be
...
captured
...
at
...
once
...
in
...
a
...
single
...
camera
...
image.
...
Widefield
...
is
...
most
...
suitable
...
for
...
2D
...
imaging
...
of
...
relatively
...
thin
...
specimens
...
because
...
all
...
the
...
depth
...
information
...
is
...
in
...
a
...
single
...
image
...
so
...
only
...
a
...
handful
...
of
...
images
...
needs
...
to
...
be
...
processed.
...
It
...
is
...
generally
...
not
...
as
...
useful
...
for
...
acquiring
...
3D
...
data
...
over
...
a
...
greater
...
depth
...
because
...
light
...
from
...
out
...
of
...
the
...
plane
...
of
...
focus
...
can
...
be
...
seen
...
as
...
blur
...
in
...
the
...
focal
...
plane.
The LMCB widefield microscopes are listed below:
Upright Microscopes
- Axio Imager (fluorescence, phase contrast, greyscale camera for sensitivity)
- Axioplan (fluorescence, differential interference contrast, greyscale camera for sensitivity)
- Axioskop 3 (phase contrast, fluorescence, colour camera for histological stains, motorised stage for tiling slides)
Inverted Microscopes
- Leica (fluorescence, phase contrast, greyscale camera for sensitivity)
- Leica 2 (fluorescence, phase contrast, colour camera for histological stains)