...
Most
...
microscopes
...
are
...
widefield
...
microscopes.
...
In
...
widefield
...
microscopy
...
the
...
entire
...
field
...
is
...
simultaneously
...
illuminated
...
throughout
...
its
...
full
...
width
...
and
...
depth
...
with
...
the
...
...
...
...
of
...
the
...
objective
...
lens
...
determining
...
how
...
much
...
of
...
the
...
specimen
...
appears
...
in
...
focus.
...
Imaging
...
and
...
processing
...
of
...
single
...
planes
...
can
...
be
...
quick
...
and
...
easy
...
because
...
an
...
entire
...
field
...
can
...
be
...
captured
...
at
...
once
...
in
...
a
...
single
...
camera
...
image.
...
Widefield
...
is
...
most
...
suitable
...
for
...
2D
...
imaging
...
of
...
relatively
...
thin
...
specimens
...
because
...
all
...
the
...
depth
...
information
...
is
...
in
...
a
...
single
...
image
...
so
...
only
...
a
...
handful
...
of
...
images
...
needs
...
to
...
be
...
processed.
...
It
...
is
...
generally
...
not
...
as
...
useful
...
for
...
acquiring
...
3D
...
data
...
over
...
a
...
greater
...
depth
...
because
...
light
...
from
...
out
...
of
...
the
...
plane
...
of
...
focus
...
can
...
be
...
seen
...
as
...
blur
...
in
...
the
...
focal
...
plane.
...
| Wiki Markup |
|---|
{float:right}!Widefield.jpg|border=0!{float}
|
The
...
LMCB
...
widefield
...
microscopes
...
are
...
listed
...
below:
...
Upright
...
Microscopes
- Axio Imager (fluorescence,
...
- phase
...
- contrast,
...
- greyscale
...
- camera
...
- for
...
- sensitivity)
...
- Axioplan (fluorescence,
...
- differential
...
- interference
...
- contrast,
...
- greyscale
...
- camera
...
- for
...
- sensitivity)
...
- Axioskop 3 (phase
...
- contrast,
...
- fluorescence,
...
- colour
...
- camera
...
- for
...
- histological
...
- stains,
...
- motorised
...
- stage
...
- for
...
- tiling
...
- slides)
...
Inverted
...
Microscopes
...
- Leica (fluorescence,
...
- phase
...
- contrast,
...
- greyscale
...
- camera
...
- for
...
- sensitivity)
...
- Leica 2 (fluorescence,
...
- phase
...
- contrast,
...
- colour
...
- camera
...
- for
...
- histological
...
- stains)
...