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The microscope has 20x and 40x long working distance objective lenses that have adjustable correction collars so the objectives can be corrected to focus through different thicknesses. For example, coverslips are usually only 170 microns thick but the growth surface of standard cell culture dishes is approximately 1 mm thick, so a correction collar would have to be set differently for specimens mounted on one or other of these surfaces.
Objective lenses
Magnification | Immersion | Designation | NA | Contrast Technique | Coverslip Correction (mm) | WD (mm) |
---|---|---|---|---|---|---|
10x | Air | N Plan | 0.25 | Ph1 | None | 17.6 |
20x | Air | N Plan LWD | 0.4 | Ph1 | 0 - 2 | 3.2 - 1.9 |
40x | Air | N Plan LWD | 0.55 | Ph2 | 0 - 2 | 3.3 - 1.9 |
20x | Air | N Plan LWD | 0.4 | LMC | 0 - 2 | 3.2 - 1.9 |
40x | Air | N Plan LWD | 0.55 | LMC | 0 - 2 | 3.3 - 1.9 |