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Filter Position

Filter Set

Excitation Filter

Dichroic Mirror

Emission Filter

Fluorophores

1

Leica A4

360/40

400

470/40

DAPI, Hoechst

2

Leica GFP

470/40

500

525/50

FITC, GFP

3

Leica N2.1

515 - 560

580

LP 590

Cy3, TRITC

4

Blank

N/A

N/A

N/A

Transmitted Light

Camera Calibrations