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Filter Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Fluorophores |
|---|---|---|---|---|---|
1 | Leica A4 | 360/40 | 400 | 470/40 | DAPI, Hoechst |
2 | Leica GFP | 470/40 | 500 | 525/50 | FITC, GFP |
3 | Leica N2.1 | 515 - 560 | 580 | LP 590 | Cy3, TRITC |
4 | Blank | N/A | N/A | N/A | Transmitted Light |
Camera Calibrations
Magnification | Binning Factor | 100 pixels = | 1 pixel = |
|---|---|---|---|
10x | 1 | 106.34 µm | 1063.4 nm |
20x | 1 | 53.17 µm | 531.7 nm |
40x | 1 | 26.59 µm | 265.9 nm |
10x | 2 | 212.68 µm | 2126.8 nm |
20x | 2 | 106.34 µm | 1063.4 nm |
40x | 2 | 53.17 µm | 531.7 nm |