Glossary of Light Microscopy Terms
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Numerical Aperture (NA)
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The higher the NA the higher the resolution of the lens. The NA cannot exceed the refractive index of the immersion medium, so lenses for use in standard immersion oils (n ≈ 1.51) tend to have a maximum NA of 1.4 to 1.45
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Numerical Aperture (Wikipedia)
Working Distance (WD)
The working distance is the distance from the front of the lens to the nearest surface of the coverslip when the specimen is in sharp focus. For objective lenses designed for use without a coverslip the surface of the culture vessel or specimen in used.
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