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What does binning mean? (Andor Technologies)
Gain
For CCD and CMOS camera gain
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Long Working Distance (LWD) objective lenses
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Many LWD objectives have correction collars that can be adjusted for a variety of specimen thicknesses. If a collar has been adjusted for a 1 mm thickness (e.g. the bottom of a plastic dish) it will not focus correctly through a glass coverslip and you will need to adjust the collar to a setting appropriate to a 170 micron thickness.
There is no systematic way of adjusting correction collars. The best way is to set the collar to approximately the correct position before observing your sample, then to focus on the sample, and finally to make fine adjustments to the collar position if necessary. The adjacent picture shows the correction collar of a Leica objective lens when adjusted for a 1 mm sample thickness. The black dot marks the position of the collar and the numbers around the objective barrel indicate the thickness for which the lens will be adjusted. The knurled ring above the dot should be used to rotate the collar. Unfortunately the Leica objectives don't have a mark for the position for a 170 micron coverslip, so the correct position can only be set approximately.
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