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Contact Andrew Vaughan for training. This machine is administered by the Light Microscopy Facility and login is through the MRCLMCB_LSM Faces Scheduling System group.
Huygens Essential on WOPR
Huygens Essential uses SVI's Maximum Likelihood Estimation (MLE) deconvolution algorithm in combination with a calculated or measured Point Spread Function (PSF) to model the way blur degrades the microscope image and then re-assigns light back to its correct focal plane. It can be used for both widefield and confocal data sets. The software has a wizard interface where the user checks or adjusts microscope parameters, measures or estimates background and signal-to-noise ratio and can apply a measured or calculated PSF before carrying out the deconvolution operation. The following options are installed in this copy:
- Widefield Deconvolution
- Confocal Deconvolution
- Spinning Disc Deconvolution
- PSF Distiller - To generate measured PSFs
- Time Series support - to automatically deconvolve time series
- Chromatic Shift Correction - To automatically correct for shifts between channels caused by aberrations and misalignments
- Various Viewers - Slicer, Twin-slicer, Ortho-slicer, Volume-renderer
Deep Thought
Processor: 2x Intel XEON CPU E5-2620, 2 GHz, 6 cores 12 logical partitions
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