Page Comparison

h1. Axio Imager
{float:right}!Axio-Imager.jpg|border=1!{float}

*Location: MRC Building, First Floor, Room 1.19.*

AxioImager is a Zeiss AxioImager upright microscope ideal for viewing  slide-mounted samples using phase contrast or fluorescence microscopy.  It has a QImaging QIClickRetiga camera designed for taking greyscale images of  fluorescent samples with high sensitivity to low light levels. All  microscope controls are manual apart from camera image capture. Image capture, whichcan isbe done through Improvision/Perkin Elmer's Openlab software. or Volocity softwares or through µManager.


To report problems or request help please email [lmcbimage@lmcb.mcbl.ucl.ac.uk|mailto:lmcbimage@lmcb.mcbl.ucl.ac.uk].

h3. Objective lenses on Axio Imager

|| Magnification || Immersion || Designation || NA || Contrast || Coverslip Correction (mm) ||
| 10X | Air | EC Plan Neofluar | 0.3 | Ph1 | 0.17 |
| 20X | Air | EC Plan Neofluar | 0.5 | Ph2 | 0.17 |
| 40X | Oil | EC Plan Neofluar | 1.3 | Ph3 | 0.17 |
| 63X | Oil | Plan Apochromat | 1.4 | Ph3 | 0.17 |
| 100X | Oil | EC Plan Neofluar | 1.3 | Ph3 | 0.17 |

h3. Fluorescence filter sets on Axio Imager

|| Filter Turret || Turret Position || Dyes || Excitation Filter || Dichroic Mirror || Emission Filter ||
| 1 | DAPI | 350/50 | 400LP | 460/50 |
| 2 | DIC | N/A | N/A | Analyser |
| 3 | Empty | N/A | N/A | N/A |
| 4 | GFP | 470/40 | 495LP | 525/50 |
| 5 | Texas Red | 560/40 | 585LP | 630/75 |
| 6 | Cy5 | 620/60 | 660LP | 665LP |

h3. Links

* [Using Volocity on the Axio Imager|^Using_Volocity_on_Axio_Imager.pdf]
* [Using Openlab to take images|^Acquisition-using-Openlab.pdf]
* [Openlab User Guide|http://cellularimaging.perkinelmer.com/pdfs/manuals/OpenlabUserGuide.pdf]
* [Volocity User Guide|http://cellularimaging.perkinelmer.com/pdfs/manuals/VolocityUserGuide.pdf]
* [Axio Imager controls|^AxioImagerControls.pdf]