Page Comparison

h1. Axio Imager

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{float:right}!Axio-Imager.jpg|border=1!{float}

*Location: MRC Building, First Floor, Room 1.19.*

The Zeiss Axio Imager upright microscope is ideal for viewing  slide-mounted specimens using phase contrast or fluorescence microscopy.  It has a QImaging Retiga greyscale camera suitable for low light level imaging of fluorescent specimens. The objective lenses are all designed for high resolution observation of specimens mounted under standard #1.5 coverslips (170 µm). All  microscope controls are manual apart from camera image capture. There are curretnlycurrently three image acquisition software packages installed on the computer: Openlab (obsolete - updates no longer available); Volocity and µManager.


To request training, report problems or ask for help please email [lmcbimage@lmcb.mcbl.ucl.ac.uk|mailto:lmcbimage@lmcb.mcbl.ucl.ac.uk].
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h3. Objective lenses

|| Magnification || Immersion || Designation || [NA|LMCBLMic:Glossary#NA] || Contrast Technique || Coverslip Correction (mm) || [WD|LMCBLMic:Glossary#WD] (mm) ||
| 10X | Air | EC Plan Neofluar | 0.3 | Ph1 | 0.17 | 5.5 |
| 20X | Air | EC Plan Neofluar | 0.5 | Ph2 | 0.17 | 2.0 |
| 40X | Oil | EC Plan Neofluar | 1.3 | Ph3 | 0.17 | 0.21 |
| 63X | Oil | Plan Apochromat | 1.4 | Ph3 | 0.17 | 0.19 |
| 100X | Oil | EC Plan Neofluar | 1.3 | Ph3 | 0.17 | 0.2 |

h3. Fluorescence filter sets

|| Filter Position || Filter Set \\ || Excitation Filter || Dichroic Mirror || Emission Filter || Fluorophores ||
| 1 | Chroma ET-DAPI | 350/50 | 400LP | 460/50 | DAPI/Hoechst |
| 2 | DIC | N/A | N/A | Analyser | DIC |
| 3 | Empty | N/A | N/A | N/A | Empty |
| 4 | Chroma ET/GFP (FITC/Cy2) | 470/40 | 495LP | 525/50 | FITC/GFP |
| 5 | Chroma ET/mCherry/Texas Red | 560/40 | 585LP | 630/75 | Texas Red |
| 6 | Chroma Custom Cy5 Longpass | 620/60 | 660LP | 665LP | Cy5/Alexa 647 |

h3. Links

* [Using Volocity on the Axio Imager|^Using_Volocity_on_Axio_Imager.pdf]
* [Using Openlab to take images|^Acquisition-using-Openlab.pdf]
* [Openlab User Guide|http://cellularimaging.perkinelmer.com/pdfs/manuals/OpenlabUserGuide.pdf]
* [Volocity User Guide|http://cellularimaging.perkinelmer.com/pdfs/manuals/VolocityUserGuide.pdf]
* [Axio Imager controls|^AxioImagerControls.pdf]