Page Comparison

h1. Axio Imager

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{float:right}!Axio-Imager.jpg|border=1!{float}

*Location: MRC Building, First Floor, Room 1.19.*

The Zeiss Axio Imager upright microscope is ideal for viewing  slide-mounted specimens using phase contrast or fluorescence microscopy.  It has a QImaging Retiga greyscale camera suitable for low light level imaging of fluorescent specimens. The objective lenses are all designed for high resolution observation of specimens mounted under standard #1.5 coverslips (170 µm). All  microscope controls are manual apart from camera image capture. There are currently three image acquisition software packages installed on the computer: Openlab (obsolete - updates no longer available); Volocity and µManager.


To request training, report problems or ask for help please email [lmcbimage@lmcb.mcbl.ucl.ac.uk|mailto:lmcbimage@lmcb.mcbl.ucl.ac.uk].
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h3. Objective lenses

|| Magnification || Immersion || Designation || [NA|LMCBLMic:Glossary#NA] || Contrast Technique || Coverslip Correction (mm) || [WD|LMCBLMic:Glossary#WD] (mm) ||
| 10X | Air | EC Plan Neofluar | 0.3 | Ph1 | 0.17 | 5.5 |
| 20X | Air | EC Plan Neofluar | 0.5 | Ph2 | 0.17 | 2.0 |
| 40X | Oil | EC Plan Neofluar | 1.3 | Ph3 | 0.17 | 0.21 |
| 63X | Oil | Plan Apochromat | 1.4 | Ph3 | 0.17 | 0.19 |
| 100X | Oil | EC Plan Neofluar | 1.3 | Ph3 | 0.17 | 0.2 |

h3. Fluorescence filter sets

|| Filter Position || Filter Set \\ || Excitation Filter || Dichroic Mirror || Emission Filter || Fluorophores ||
| 1 | Chroma ET-DAPI | 350/50 | 400LP | 460/50 | DAPI/Hoechst |
| 2 | DIC | N/A | N/A | Analyser | DIC |
| 3 | Empty | N/A | N/A | N/A | Empty |
| 4 | Chroma ET/GFP (FITC/Cy2) | 470/40 | 495LP | 525/50 | FITC/GFP |
| 5 | Chroma ET/mCherry/Texas Red | 560/40 | 585LP | 630/75 | Texas Red |
| 6 | Chroma Custom Cy5 Longpass | 620/60 | 660LP | 665LP | Cy5/Alexa 647 |

Note that the ET filter sets are extremely efficient and transmit more light than older filter sets. This goes for the excitation filters as well as the emission filters, so it is possible for specimens to photobleach rather quickly. Please use the intensity dial on the Prior Lumen light source to reduce the excitation intensity if bleaching is a problem.

h3. Links

* [Using Volocity on the Axio Imager|^Using_Volocity_on_Axio_Imager.pdf]
* [Using Openlab to take images|^Acquisition-using-Openlab.pdf]
* [Openlab User Guide|http://cellularimaging.perkinelmer.com/pdfs/manuals/OpenlabUserGuide.pdf]
* [Volocity User Guide|http://cellularimaging.perkinelmer.com/pdfs/manuals/VolocityUserGuide.pdf]
* [Axio Imager controls|^AxioImagerControls.pdf]