Axio Imager
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h1. Axio Imager ---- {float:right}!Axio-Imager.jpg|border=1!{float} * |
Location:
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MRC
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Building,
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First
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Floor,
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Room
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1.19.
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The
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Zeiss
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Axio
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Imager
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upright
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microscope
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is
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ideal
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for
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viewing
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slide-mounted
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specimens
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using
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phase
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contrast
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or
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fluorescence
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microscopy.
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It
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has
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a
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QImaging
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Retiga
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greyscale
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camera
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suitable
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for
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low
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light
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level
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imaging
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of
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fluorescent
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specimens.
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The
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objective
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lenses
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are
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all
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designed
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for
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high
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resolution
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observation
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of
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specimens
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mounted
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under
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standard
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#1.5
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coverslips
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(
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170 µm).
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All
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microscope
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controls
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are
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manual
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apart
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from
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camera
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image
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capture.
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There
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are
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currently
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three
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image
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acquisition
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software
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packages
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installed
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on
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the
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computer:
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Openlab
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(obsolete
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-
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updates
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no
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longer
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available);
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Volocity
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and
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µManager.
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To
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request
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training,
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report
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problems
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or
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ask
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for
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help
...
please
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...
...
.
| Table of Contents | ||
|---|---|---|
|
Objective lenses
Magnification | Immersion | Designation | Contrast Technique | Coverslip Correction (mm) | WD (mm) | |
|---|---|---|---|---|---|---|
10X | Air | EC Plan Neofluar | 0.3 | Ph1 | 0.17 | 5.5 |
20X | Air | EC Plan Neofluar | 0.5 | Ph2 | 0.17 | 2.0 |
40X | Oil | EC Plan Neofluar | 1.3 | Ph3 | 0.17 | 0.21 |
63X | Oil | Plan Apochromat | 1.4 | Ph3 | 0.17 | 0.19 |
100X | Oil | EC Plan Neofluar | 1.3 | Ph3 | 0.17 | 0.2 |
Fluorescence filter sets
Filter Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Fluorophores |
|---|---|---|---|---|---|
1 | Chroma ET-DAPI | 350/50 | 400LP | 460/50 | DAPI/Hoechst |
2 | DIC | N/A | N/A | Analyser | DIC |
3 | Empty | N/A | N/A | N/A | Empty |
4 | Chroma ET/GFP (FITC/Cy2) | 470/40 | 495LP | 525/50 | FITC/GFP |
5 | Chroma ET/mCherry/Texas Red | 560/40 | 585LP | 630/75 | Texas Red |
6 | Chroma Custom ET Cy5 Longpass | 620/60 | 660LP | 665LP | Cy5/Alexa 647 |
Note that the ET filter sets are extremely efficient and transmit more light than older filter sets. This goes for the excitation filters as well as the emission filters, so it is possible for specimens to photobleach rather quickly. Please use the intensity dial on the Prior Lumen light source to reduce the excitation intensity if bleaching is a problem.