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Upright fluorescence microscope with phase contrast
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Objective lenses
Magnification | Immersion | Designation | Contrast Technique | Coverslip Correction (mm) | WD (mm) | |
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10X | Air | EC Plan Neofluar | 0.3 | Ph1 | 0.17 | 5.5 |
20X | Air | EC Plan Neofluar | 0.5 | Ph2 | 0.17 | 2.0 |
40X | Oil | EC Plan Neofluar | 1.3 | Ph3 | 0.17 | 0.21 |
63X | Oil | Plan Apochromat | 1.4 | Ph3 | 0.17 | 0.19 |
100X | Oil | EC Plan Neofluar | 1.3 | Ph3 | 0.17 | 0.2 |
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Filter Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Fluorophores |
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1 | Chroma ET-DAPI | 350/50 | 400LP | 460/50 | DAPI/Hoechst |
2 | DIC | N/A | N/A | Analyser | DIC |
3 | Empty | N/A | N/A | N/A | Empty |
4 | Chroma ET/GFP (FITC/Cy2) | 470/40 | 495LP | 525/50 | FITC/GFP |
5 | Chroma ET/mCherry/Texas Red | 560/40 | 585LP | 630/75 | Texas Red |
6 | Chroma Custom ET Cy5 Longpass | 620/60 | 660LP | 665LP | Cy5/Alexa 647 |
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