Leica DMIRB (room 1.19)
Objective Lenses
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Magnification | Immersion | Designation | NA | Contrast | Coverslip Correction (mm) | WD (mm) |
|---|
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10X |
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Air |
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N Plan |
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0.25 |
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Ph1 |
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None |
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17.6 |
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20X | Air | HCX PL Fluotar LWD | 0.4 | Ph1 | 0 - 2 | 6.9 |
40X | Air | N Plan LWD | 0.55 | Ph2 | 0 - 2 | 3.3 - 1.9 |
10X | Air | C Plan | 0.22 | LMC | None | 7.8 |
20X | Air | N Plan LWD | 0.4 | LMC | 0 - 2 | 3.2 - 1.9 |
40X | Air | N Plan LWD | 0.55 | LMC | 0 - 2 | 3.3 - 1.9 |
NA = Numerical Aperture, WD = Working Distance, LWD = Long Working Distance, LMC = Leica Modulation Contrast
Fluorescence Filter Sets
Filter |
|---|
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Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Fluorophores |
|---|---|---|---|---|---|
1 | Leica A4 | BP 360/40 |
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400 |
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BP 470/40 |
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DAPI, Hoechst |
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2 | Leica GFP | BP 470/40 | 500 | BP 525/50 | FITC, GFP |
3 | Leica N2.1 | BP 515-560 | 580 | LP 590 | Cy3, TRITC |
4 | Blank | N/A | N/A | N/A | Transmitted Light |