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Advanced microscopy training is compulsory for anyone wanting to carry out the following techniquestechniques below but can be requested by anyone who wants further training after the introductory session:
- Differential Interference Contrast (DIC aka. Nomarski Contrast) on confocal microscopes
- Imaging of samples in non-standard or home-built specimen holders
- Live imaging of any kind
- Photo-bleaching, photo-activation and photo-conversion techniques (e.g. FRAP, FLIP, etc)
- Fluorescence Resonance Energy Transfer (FRET)
- Techniques requiring the use of a motorised stage (e.g. multi-position time-lapse, tiling/stitching)
Advanced training will consist of a session where the user will operate the machine with one of their own specimens under the supervision of a member of the light microscopy facility. It is likely that live specimens in particular will require specific stage adapters and environmental conditions and users must discuss this with their tutor in advance give advanced warning so the necessary arrangements can be made. It is also important that you check with the light microscopy facility whether your particular procedure or specimen can be accommodated using the current equipment in the facility. Not all specimens or techniques will necessarily be possible.