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Advanced microscope training is compulsory for anyone wanting to carry out the techniques below but can be requested by anyone who wants further training after the introductory session. You will need an advanced session if you are doing any of the following techniques:
- Differential Interference Contrast (DIC aka. Nomarski Contrast) on confocal microscopes
- Imaging of samples in non-standard or home-built specimen holders
- Live imaging of any kind
- Photo-bleaching, photo-activation and photo-conversion techniques (e.g. FRAP, FLIP, etc)
- Fluorescence Resonance Energy Transfer (FRET)
- Techniques requiring the use of a motorised stage (e.g. multi-position time-lapse, tiling/stitching)
Advanced training will consist of a session where the user you will operate the machine microscope with one of their your own specimens under the supervision of a member of the light microscopy facility. It is likely that some specimens (live specimens in particular) will require specific stage adapters and environmental conditions and users must give advanced warning so so please give details of the specimen to the staff member who will be supervising you so that the necessary arrangements can be made. It is also important that users you check with the light microscopy facility to find out whether your particular procedure or specimen can be accommodated using the current equipment in the facility.