Microscope Stand Information
Unless otherwise stated the microscope objective lenses require specimens to be mounted on a #1.5 glass coverslip (170 microns) or in a chamber/dish with a coverslip bottom of the same thickness.
Widefield
Use a widefield microscope if your specimen is thin and relatively free of out-of focus blur or you don't need to optically section it for processing, analysis and visualisation in 3D.
Microscope | Upright or Inverted? | Objective Lenses | Fluorescence Colours | Camera | Automation | Techniques |
|---|---|---|---|---|---|---|
Axioplan | Upright | 10x air to 100x oil | Blue, Green, Red, Far-Red | Greyscale high sensitivity CCD | Manual | Good for high sensitivity and high resolution fluorescence microscopy and differential interference contrast (DIC) of fixed specimens on slides |
Axio Imager | Upright | 10x air to 100x oil | Blue, Green, Red, Far-Red (Cyan and Yellow available on request) | Greyscale high sensitivity CCD | Manual | Good for high sensitivity and high resolution fluorescence microscopy and phase contrast of fixed specimens on slides |
Axioskop 3 | Upright | 10x air to 100x oil | Blue, Green, Red | Colour CCD | Motorised | Good for moderate to high resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides. Supports tiling and stitching. |
Inverted | 10x air to 40x LWD* | Blue, Green, Red | Greyscale high sensitivity CCD | Manual | Good for moderate resolution fluorescence microscopy and phase contrast of fixed/live specimens on slides or in dishes and plates with glass or plastic bottoms | |
Leica 2 | Inverted | 10x air to 40x LWD* | Blue, Green, Red | Colour CCD | Manual | Good for moderate resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides or in dishes and plates with glass or plastic bottoms |
Confocal
Use a confocal if your specimen in thick, has significant out-of-focus blur, or if you need to process, analyse or visualise it in 3D.
Microscope | Upright or Inverted? | Objective Lenses | Lasers | Detectors | Simultaneous/Sequential | Manual/Automated Stage | Climate Control | Techniques |
|---|---|---|---|---|---|---|---|---|
Radiance | Upright | 10x air to 60x oil (water dipping lenses available) | Argon, 543, 635 | 3x GaAsP | Simultaneous and Sequential | Manual | Available (heat only) | Imaging of specimens on slides and in gasket sealed chambers or open dishes using dipping lenses. DIC. FRAP. |
SPE | Upright | 10x air to 63x oil (water dipping lenses available) | 405, 488, 532, 640 | 1x PMT | Sequential only | Manual | Available | Imaging of specimens on slides and live specimens in Warner Instruments perfusion chamber using dipping lenses. DIC. Spectral imaging. |
SPE | Upright | 10x air to 63x oil (water dipping lenses available) | 405, 488, 532, 640 | 1x PMT | Sequential only | Manual | Available | Imaging of specimens on slides and live specimens in open dishes using Oko-Lab incubation equipment and dipping lenses. DIC. Spectral imaging. |
SPE3 | Inverted | 10x air to 63x oil | 405, 488, 561, 640 | 1x PMT | Sequential only | Automated | No | Imaging of specimens on slides and in dishes. DIC. Spectral Imaging. Multi-point and stitching. Matrix Screener. |
SP5 | Inverted | 10x air to 63x oil (63x glycerol available) | 405, Argon, 561, 594, 633 | 1x PMT and 3x Hybrids | Simultaneous and Sequential | Automated | Available | Imaging of specimens on slides and in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Spectral imaging. DIC. Multi-point and stitching. FRAP. FRET. Live Data Mode. Matrix Screener. |
Vox | Inverted | 10x air to 100x oil (60x water immersion available) | 405, 488, 561, 640 | 1x EMCCD camera | Sequential only | Automated | Available | Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Solent Scientific cage type incubation chamber. DIC. Multi-point and stitching. FRAP. |
Time-lapse inverted microscope systems
Microscope | Objective Lenses | Fluorescence Colours | Camera | Techniques |
|---|---|---|---|---|
GFP1 | 10x to 40x air and LWD (oil lenses available) | Green and Red | Greyscale high sensitivity CCD | Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. Stage adapters support Nunc and Mat-Tek multi-well plates and 35 mm Mat-Tek dishes. If you need to use another vessel or adapter you must contact Andrew Vaughan |
GFP2 | 10x to 40x air and LWD (oil lenses available) | Blue, Green and Red | Greyscale high sensitivity CCD | Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. Stage adapters support Nunc and Mat-Tek multi-well plates and 35 mm Mat-Tek dishes. If you need to use another vessel or adapter you must contact Andrew Vaughan |
N-STORM (coming soon) | 10x air to 100x oil TIRF lens | Blue, Green, Red and Far-Red | Greyscale ultra-high sensitivity EMCCD | Optimally configured for high resolution and high sensitivity fluorescence microscopy of living specimens in XYZT for deconvolution, TIRF and super-resolution using N-STORM and dSTORM. |