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- Always remove latex or nitrile gloves before touching the keyboard, mouse, microscope focus and buttons to prevent contamination with biological material and immersion oil
- Always keep any immersion oil bottles in the petri dishes provided. This is to stop oil getting everywhere. Do not put anything else in the dishes
- Always keep stage adaptors, inserts and relevant immersion oils inside the chamber so they stay at the same temperature as the microscope
- You If you need to calibrate the stage you MUST make sure that the objective lens nose piece is in its lowest position before calibrating the stagefirst. If this is not done the stage will hit the condenser or an objective lens when calibrating
- You must NEVER leave the microscope stand with the objective lens nose piece in the Escape (Nikon) or Load (Zeiss) position. This is because the next user will not be able to focus with the Escape or Load button pressed. Also, if they work out what has happened and press the refocus button the objective lens may go straight through their sample
- Always check carefully for any spilt medium on or around the microscope stage, nose piece and inside the chamber. Clean up any spills. The salts in media react with the metal of the microscope and can cause serious damage
- You MUST dispose of your specimens according to the LMCB Code of Practice for Laboratory Workers. Gloves and tissues should be discarded in the yellow clinical waste bag. Sharps and small biological specimens (e.g. Lab-Teks and 35 mm dishes) may be placed in the sharps bin provided, but any larger biological specimens must be taken away and treated overnight with a suitable disinfectant before disposal.