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Table of Contents

Objective Lenses

...

Magnification

Immersion

Type

NA

Contrast Technique

Coverslip Correction (mm)

WD (mm)

...

10X

...

Air

...

N Plan

...

0.25

...

Ph1

...

None

...

17.6

...

20X

Air

HCX PL Fluotar LWD

0.4

Ph1

0 - 2

6.9

40X

Air

N Plan LWD

0.55

Ph2

0 - 2

3.3 - 1.9

10X

Air

C Plan

0.22

LMC

None

7.8

20X

Air

N Plan LWD

0.4

LMC

0 - 2

3.2 - 1.9

40X

Air

N Plan LWD

0.55

LMC

0 - 2

3.3 - 1.9

Also available...

40x

Water

HCX PL APO

1.1

N/A

0.14 - 0.18

0.63

NA = Numerical Aperture, WD = Working Distance, LWD = Long Working Distance, LMC = Leica Modulation Contrast

Fluorescence Filter Sets

Filter Position

Filter Set

Excitation Filter

...

Dichroic Mirror

Emission Filter

Fluorophores

1

Leica A

BP 340-380

400

LP 425

DAPI, Hoechst, transmitted light

2

Leica

GFP

L5

BP

470

480/40

500

505

BP

525

527/

50

30

FITC, GFP

3

Leica N2.1

BP 515-560

580

LP 590

Cy3, TRITC

4

Blank

N/A

N/A

N/A

Transmitted Light

Chroma ET-eCFP

436/20

455

480/40

eCFP