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MICROSCOPE: Nikon N-STORM based on ECLIPSE Ti-E inverted microscope stand
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STORM RESOLUTION | |
|---|---|
XY | ~ 20 - 30 nm |
Z | ~ 50 - 75 nm |
3D STORM axial range | ± 500 nm |
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IMAGING MODES
- STORM (2D or 3D using cylindrical lens)
Multiparameter widefield with laser or LED illumination (multi-colour, multi-point, time-lapse)
- TIRF
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LIGHT SOURCES | ||
|---|---|---|
Light Source | Type | Wavelengths [nm] |
Nikon LU-NV laser unit | Laser | 405, 488, 561, 647 |
Lumencor SPECTRA Light Engine® | LED | 395, 440, 470, 508, 555, 640 |
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OBJECTIVE LENSES | |||||
|---|---|---|---|---|---|
Objective | Magnification | Immersion Medium | Numerical Aperture | Coverslip Correction [mm] | Working Distance [mm] |
CFI Plan Fluor 10x | 10x | air | 0.3 | 0.17 | 16 |
CFI Plan Fluor 40x oil | 40x | oil | 1.3 | 0.17 | 0.2 |
CFI Plan Apo VC 60x WI | 60x | water | 1.2 | 0.15 – 0.18 | 0.31 – 0.28 |
CFI Plan Apo VC 60x oil | 60x | oil | 1.4 | 0.17 | 0.13 |
CFI Apo TIRF 100X Oil | 100x | oil | 1.49 | 0.13 – 0.19 (23°C) 0.14 (37°C) | 0.12 |
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FLUORESCENCE FILTER SETS | ||
|---|---|---|
Turret Position | Emission Filter | Example Fluorophores |
1 | 49000 | DAPI |
2 | 49002 | GFP |
3 | 49008 | mCherry |
4 | C-S2 | Quadband compatible with all Lumencore lines |
5 | C-STO | Quadband compatible with all Agilent laser lines |
6 | N-STO | Alexa Fluor 647, Cy5 |
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CAMERAS
Andor iXon DU897E (EMCCD) for STORM imaging (right side port)
Andor Technology sCMOS Zyla 4.2 for widefield acquisitions
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INCUBATION
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: Solent Scientific chamber heater and CO2 supply
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unit
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SOFTWARE
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: NIS-Elements Ar including NIS-A N-STORM analysis module
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ADDITIONAL INFORMATION
- anti-vibration optical table
- fully enclosed
- perfect focus system (PFS)
- motorized XY stage with encoders
- piezo Z stage