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Objective Lenses
Magnification | Immersion | DesignationType | NA | Contrast Technique | Coverslip Correction (mm) | WD (mm) |
|---|---|---|---|---|---|---|
10X | Air | N Plan | 0.25 | Ph1 | None | 17.6 |
20X | Air | HCX PL Fluotar LWD | 0.4 | Ph1 | 0 - 2 | 6.9 |
40X | Air | N Plan LWD | 0.55 | Ph2 | 0 - 2 | 3.3 - 1.9 |
10X | Air | C Plan | 0.22 | LMC | None | 7.8 |
20X | Air | N Plan LWD | 0.4 | LMC | 0 - 2 | 3.2 - 1.9 |
40X | Air | N Plan LWD | 0.55 | LMC | 0 - 2 | 3.3 - 1.9 |
Also available... | ||||||
40x | Water | HCX PL APO | 1.1 | N/A | 0.14 - 0.18 | 0.63 |
NA = Numerical Aperture, WD = Working Distance, LWD = Long Working Distance, LMC = Leica Modulation Contrast
Fluorescence Filter Sets
Filter Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Fluorophores | ||||
|---|---|---|---|---|---|---|---|---|---|
1 | Leica | A4A | BP | 360/40340-380 | 400 | BP 470/40LP 425 | DAPI, Hoechst, transmitted light | ||
2 | Leica | GFPL5 | BP | 470480/40 | 500505 | BP | 525527/ | 5030 | FITC, GFP |
3 | Leica N2.1 | BP 515-560 | 580 | LP 590 | Cy3, TRITC | ||||
4 | Blank | N/A | N/A | N/A | Transmitted LightChroma ET-eCFP | 436/20 | 455 | 480/40 | eCFP |