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Use a widefield microscope if your specimen is thin and relatively free of out-of focus blur; or you don't need to optically section it for processing, analysis and visualisation in 3D.
Microscope Name | Upright or Inverted? | Fluorescence Colours | Key Features | Techniques |
|---|---|---|---|---|
Axioplan | Upright | Blue, Green, Red, Far-Red | Zeiss Axioplan 2 microscope . QImaging QClick camera . Fluorescence . DIC. | High sensitivity and high resolution fluorescence microscopy and differential interference contrast (DIC) of fixed specimens on slides |
Axio Imager | Upright | Blue, Green, Red, Far-Red. Cyan and Yellow available on request | Zeiss Axio Imager A1 microscope . QImaging Retiga EXi camera . Fluorescence . Phase contrast | High sensitivity and high resolution fluorescence microscopy and phase contrast of fixed specimens on slides |
Axioskop 3 | Upright | Blue, Green, Red | Zeiss Axioskop microscope . QImaging QICAM colour camera . Fluorescence . Phase contrast . Motorised stage | Moderate to high resolution fluorescence microscopy and imaging of histologically or histochemically stained specimens on slides. Motorised stage supports tiling and stitching of specimens. |
Inverted | Blue, Green, Red | Leica DMIRB microscope . Hamamatsu Orca NRB camera . Fluorescence . Phase contrast . Long distance lenses suitable for use with normal TC dishes and plates | Good for moderate resolution fluorescence microscopy and phase contrast of fixed/live specimens on slides, or in dishes and plates with glass or plastic bottoms. | |
Leica 2 | Inverted | Blue, Cyan, Green, Red | Leica DMIRB microscope . Leica DFC7000T colour camera . Fluorescence . Phase contrast . Leica Modulation Contrast (LMC) . Long distance lenses suitable for use with normal TC dishes and plates | Moderate to high resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides, or in dishes and plates with glass or plastic bottoms. Leica Modulation Contrast (LMC) lenses for brightfield contrast imaging that is less sensitive to the meniscus in well plates. Cyan filter cube for CFP. |
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Microscope | Upright or Inverted? | Fluorescence Colours | Key Features | Live Imaging | Techniques |
|---|---|---|---|---|---|
GFP1 | Inverted | Green, Red, Far-Red | Zeiss Axio Observer Z1 stand with Hamamatsu Orca Flash4.0 v2 sCMOS camera. Fully automated with motorised stage. Fluorescence and phase contrast. | Solent Scientific chamber with adaptor for multi-well plate. Other adaptors available on request. | Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. |
GFP2 | Inverted | Cyan, Green, Red, Far-Red | Zeiss Axiovert 200M stand with Hamamatsu Orca ER CCD camera. Fully automated with motorised stage. Fluorescence and phase contrast. Long distance lenses. | Solent Scientific chamber with adaptor for multi-well plate. Other adaptors available on request. | Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. Long distance lenses suitable for use with normal TC dishes and plates |
GFP3 | Inverted | Blue, Green and Red. Cyan and Yellow available on request. | Nikon TiE stand. DS-Qi2 sCMOS camera with wide field of view. Fully automated with motorised stage. Perfect Focus System. Fluorescence and phase contrast. Long distance lenses. | Oko-Lab cage incubator with adaptors for multi-well plates, 35 mm dishes and chambered coverslips. | Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. Long distance lenses suitable for use with normal TC dishes and plates. Perfect Focus System ensures that the sample remains in focus over long periods. |
Super-resolution Microscopes
| Microscope | Upright or Inverted | Laser Lines | Key Features | Live Imaging | Techniques |
|---|---|---|---|---|---|
| Elyra | Inverted | 405, 488, 561, 642 | Zeiss Elyra PS.1 with Axio Observer Z1 stand. Andor iXon Du-897 EMCCD for PALM. PCO Edge sCMOS for SIM. Motorised stage and piezo focus. Single-band filter turret and optional dual-band filter turret for high speed dual channel imaging. 3D SIM and 3D PALM capabilities. | Tokai-Hit stage-top incubator with adaptors suitable for 35 mm dishes and chambered coverslips. | Structured Illumination Microscopy, PALM and dSTORM, Total Internal Reflection (TIRF) microscopy |
| GFP4 | Inverted | 405, 488, 561, 642 | Visitech vt-iSIM and Nikon TiE stand. Hamamatsu Orca Flash4.0 v3 camera for vt-iSIM. Andor Zyla for wide-field imaging. Motorised stage and piezo focus. | Oko-Lab cage incubator with adaptors for multi-well plates, 35 mm dishes and chambered coverslips. | Instant SIM that gives a live structured illumination image without processing. Further resolution possible with deconvolution. Wide-field. |
| N-STORM | Inverted | 405, 488, 561, 647 | Nikon N-STORM and Ti stand. Andor iXon Du-897 EMCCD camera for STORM and dSTORM. Astigmatic lens for 3D STORM. Zyla sCMOS camera for wide-field imaging. | Solent Scientific cage incubator with adaptors for multi-well plates and 35 mm dishes. | Stochastic Optical Reconstruction Microscopy (STORM) and direct STORM. TIRF microscopy. Wide-field time-lapse. |
| STED | Inverted | 405, WLL | Leica TCS gSTED 3X with DMI6000B stand. 3x Hybrid and 2x PMT detectors. Motorised stage. High speed Z Galvo focus. 532, 660 and 775 nm depletion lasers for multi-colour STED. 3D STED capability. | Life Imaging Services Box cage incubator system with adaptors for 35 mm dishes and chambered coverslips | Multi-colour STED and confocal microscopy. 3D STED. Tiling of large specimens. |
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